|Name of the Enzyme
||Role in DNA Replication
|1. Endonuclease (incision enzyme)
||Causes nick (break) in the DNA strand at the point of origin.
|2. DNA helicase (also called rep protein or DNA unwinding protein)
||Brings about unwinding of the two strands of DNA molecule (This enzyme needs ATP energy for unwinding).
|3. DNA gyrase (topoiosomerase)
||Removes the supercoils in the DNA double helix which are developed ahead of the replication fork during the unwinding.
|4. SSBP (single Strand DNA Binding Protein)also called SSB or Helix destabilizing protein).
||Prevents the separated strands of the DNA molecules from coiling around each other again.
|5. DNA primase
||Catalyses synthesis of RNA primer
|6. DNA polymerase (DNA replicases).
||These enzymes bring about synthesis of new strand of DNA on each old template strand.
|7. DNA ligase (Poynucleotide ligases).
||These enzymes are responsible for joining the Okazaki fragments of the new lagging strand during replication.