MAJOR STAGES INVOLVED IN MICROPROPAGATION

MAJOR STAGES INVOLVED IN MICROPROPAGATION

STAGE                                   METHOD INVOLVED

STAGE 0                                Selection of mother plant and its maintenance

STAGE  I                                Initiation and establishment of culture.

STAGE II                                Multiplication of shoots or rapid somatic

                                                embryo formation.

STAGE III                             In vitro germination of somatic embryos and/or

                                                  rooting of shoots

STAGE  IV                             Transfer of plantlets to sterilized soil for

                                                hardening under greenhouse environment.