CHROMATOGRAPHIC TECHNIQUES ALONG WITH THE PRINCIPLES FOR SEPARATION OF PROTEINS.

CHROMATOGRAPHIC TECHNIQUES ALONG WITH THE PRINCIPLES FOR SEPARATION OF PROTEINS.

CHROMATOGFRAPHY                                    PRINCIPLE

Gel filtration (size exclusion)                              Size and Shape.

Ion-exchange                                                        Net Charge

Chromatofocussing                                              Net Charge

Affinity                                                                Biological affinity and

                                                                             Molecular recognition.

Hydrophobic Interaction                                      Polarity (hydrophobicity

                                                                             Of molecules)

 

Immobilized metal-ion affinity                             Metal- ion binding.